TABLE 1.
Statistics of the 10 lowest energy structures
| Number of restraints used in structure calculations | |
| NOE distance | 218 |
| Experimentally derived | 113 |
| (residues 7–17, 28–35) | 99 |
| (intranucleotide) | 36 |
| (intermolecular RNA-GTP) | 14 |
| Total involving exchangeable protons | 47 |
| Model-derived A-form stems | |
| (residues 1–6, 18–20, 25–27, 36–41) | 77 |
| Model-derived UUCG tetraloop | |
| (residues 21–24) | 28 |
| Dihedral angle | 149 |
| Dipolar couplings | 25 |
| Stem hydrogen bonds | 42 |
| Total no. of restraints | 434 |
| Average rmsd from experimental restraints | |
| Distance (no violations >0.2 Å) | 0.02 Å |
| Dihedral (no violations >5°) | 0.14° |
| RDC (no violations >5 Hz) | 1.6 Hz |
| Average rmsd from ideal geometries | |
| Bonds | 0.006 Å |
| Angles | 1.21° |
| Impropers | 0.74° |
| Heavy atom rmsd from mean structure | |
| All residues (1–41, GTP) | 3.38 ± 0.77 Å |
| Recognition bulge and ligand (residues 8–16, 29–34, GTP) | 1.97 ± 0.34 Å |
| Recognition bulge and ligand without | 1.47 ± 0.38 Å |
| unconserved U’s (see text) (residues 8, 9, 11, 12, 14–16, 29, 31–34, GTP) Recognition bulge alone without | 1.47 ± 0.36 Å |
| unconserved U’s (see text) (residues 8, 9, 11, 12, 14–16, 29, 31–34) Ligand alone (GTP) | 1.35 ± 0.37 Å |
