RNA polymerase II CTD phosphopeptides compete with RNA for the interaction with Pcf11

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FIGURE 3.
FIGURE 3.

Binding of the RNA oligos to Pcf11 monitored by SPR. (A) Overlap of three SPR curves obtained by the introduction of Pcf11-CID to immobilized GA-RNA, CU-RNA, and GA/CU-RNA. (B) The increase in response units resulting from each addition of Pcf11-CID titration plotted against protein concentration. The curve is the line of best fit using an expression for a 1:1 binding model.

This Article

  1. Published in Advance February 22, 2006, doi: 10.1261/rna.2304506 RNA 12: 555-560

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