Pyrrolo-C as a fluorescent probe for monitoring RNA secondary structure formation

TABLE 1.

Relative fluorescence intensities and anisotropies of free PC, ssRNA, and dsRNA

Fluorescence intensity
Buffer (%) 50% ETOH (%) 50% Formamide Anisotropy
Free PC 100 ± 1 353 ± 1 216 ± 2 0.06 ± 0.02
ssRNA 48 ± 2 85 ± 2 162 ± 3 0.13 ± 0.01
dsRNA 31 ± 4 38 ± 2 98 ± 1 0.20 ± 0.02

This Article

  1. Published in Advance January 23, 2006, doi: 10.1261/rna.2165806 RNA 12: 522-529

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