Pyrrolo-C as a fluorescent probe for monitoring RNA secondary structure formation

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FIGURE 4.
FIGURE 4.

The relative fluorescence intensity of free pyrrolo-C ribonucleoside (gray triangles), ssRNA (filled circles), and dsRNA (open circles) as a function of pH (A) and (v/v)% content (B) of formamide in standard buffer (50 mM Tris, 25 mM MES-NaOH, 25 mM acetic acid–NaOH at pH 7.5, 100 mM NaCl) at 25°C. Errors are derived from at least two independent measurements.

This Article

  1. Published in Advance January 23, 2006, doi: 10.1261/rna.2165806 RNA 12: 522-529

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