Pyrrolo-C as a fluorescent probe for monitoring RNA secondary structure formation

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FIGURE 3.
FIGURE 3.

Relative fluorescence intensity of free pyrrolo-C ribonucleoside (gray triangles), ssRNA (filled circles), and dsRNA (open circles) as a function of NaCl (A) and MgCl2 (B) concentration in 50 mM Tris, 25 mM MES-NaOH, and 25 mM acetic acid–NaOH (pH 7.5; initially containing ~12 mM Na+) at 25°C. Errors are derived from at least two independent measurements.

This Article

  1. Published in Advance January 23, 2006, doi: 10.1261/rna.2165806 RNA 12: 522-529

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