TABLE 2.
Plasmids
| Name | Description | Reference |
|---|---|---|
| B254 | 3.1-kb SspI/PmeI fragment of TRF4 subcloned from pSup1 into SspI/PmeI YCpLac33 | Kadaba et al. 2004 |
| B256 | 3.1-kb SacI/PstI fragment of TRF4 from B254 into SacI/PstI YEpLac195 | Kadaba et al. 2004 |
| B315 | 3.1-kb TRF4 gene harboring trf4-236 mutation was generated by quick change mutagenesis using B256 as template | This study |
| B316 | KpnI/PstI digested 3.1-kb fragment of trf4-236 from B315 subcloned into KpnI/PstI YCpLac33 | This study |
| pΔBE6 | snr6 Δ59–72 | Eschenlauer et al. 1993 |
| B288 | 1.4-kb SphI/EcoRI fragment of SNR6 subcloned from PΔBE6 into SphI/EcoRI YCpLac111 | This study |
| CB1455 | 3.1-kb PstI/BamHI fragment of TRF5 cloned into PstI/BamHI PRS315 | Dr. Michael Christman |
| B232 | PstI/BamHI 3.1-kb fragment of TRF5 from CB1455 | This study |
| subcloned into PstI/BamHI YEpLac195 | This study | |
| B180 | YCpLac111: CEN4, LEU2 cloning vector | Gietz and Sugino 1988 |
| B181 | YCpLac33: CEN4, URA3 cloning vector | Gietz and Sugino 1988 |
| B184 | YEpLac195: URA3 high copy vector | Gietz and Sugino 1988 |
