Nuclear RNA surveillance in Saccharomyces cerevisiae: Trf4p-dependent polyadenylation of nascent hypomethylated tRNA and an aberrant form of 5S rRNA

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FIGURE 5.
FIGURE 5.

Polyadenylated pre-tRNAiMet contains the 5′ leader sequence. (A) Northern hybridization analysis of total RNA (5 μg) and poly(A)+ RNA (2–4 μg) from trm6-504 (Y190) and wild type (Y200). (B) Northern hybridization of total RNA (5 μg) and poly(A)+ RNA (2–4 μg) from trm6-504 rrp6Δ (Y298) with (+) or without (−) pretreatment with oligo d(T) and RNaseH. The blots were hybridized with radiolabeled probes (JA382, JA72, JA66, and JA48) that recognize only pre-tRNAiMet containing the 5′ leader (A,B, lanes 1–3) or a radiolabeled probe (JA11) that is complementary to an internal portion of tRNAiMet (B, lanes 4–6). The point of migration of pre-tRNAiMet and mature tRNAiMet are shown for clarity.

This Article

  1. Published in Advance January 23, 2006, doi: 10.1261/rna.2305406 RNA 12: 508-521

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