Nuclear RNA surveillance in Saccharomyces cerevisiae: Trf4p-dependent polyadenylation of nascent hypomethylated tRNA and an aberrant form of 5S rRNA

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FIGURE 4.
FIGURE 4.

Precursor form of hypomodified tRNAiMet is being polyadenylated. (A) An abbreviated and stylized representation of the tRNAiMet coding sequence with the predicted 3′ ends of each pre-tRNAiMet synthesized from IMT1–4 shown. The predicted transcription termination sites of pre-tRNAiMet are shown as 5–7 tandem Ts (Us in the transcript) are shown in bold. (B) The abbreviated coding sequence is followed by the exact sequence found at the 3′ end of each cDNA, and the poly(A) tail lengths are shown as a range (16–30), but the majority of the poly(A) tails were 16–19 As in length. The number of clones with the identical 3′ end sequence are shown in parentheses. (C) The abbreviated coding sequence for the truncated cDNAs found end with the last nucleotide of the tRNA (C65 or C60) followed by the poly(A) tail length for each.

This Article

  1. Published in Advance January 23, 2006, doi: 10.1261/rna.2305406 RNA 12: 508-521

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