A general two-step strategy to synthesize lariat RNAs

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FIGURE 5.
FIGURE 5.

Syntheses of ai5γ group II intron lariat RNA. The two-step RNA substrates include a stem to favor natural lariat formation. For the one-step synthesis and for the first step of the two-step synthesis, t = 0, 0.5, and 1.5 h using the 7S11 deoxyribozyme. For the second step of the two-step synthesis, t = 10 and 60 min using T4 RNA ligase (– denotes PAGE-purified branched RNA incubated without T4 RNA ligase). Samples were analyzed by 5% PAGE.

This Article

  1. Published in Advance December 22, 2005, doi: 10.1261/rna.2259406 RNA 12: 313-321

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