A general two-step strategy to synthesize lariat RNAs

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FIGURE 3.
FIGURE 3.

Formation of natural and unnatural lariat RNA isomers in the T4 RNA ligase loop-closure reaction, and the blocking and capping approaches to control which isomer is formed. On each structure is marked the cleavage site for debranching enzyme Dbr1p, which is used to characterize the lariats (Fig. 4).

This Article

  1. Published in Advance December 22, 2005, doi: 10.1261/rna.2259406 RNA 12: 313-321

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