Effect of heparin on RNA synthesis by Δ21 from linear and circular templates. RNA synthesis over time was examined with templates +1C-L and +1C-C. Heparin (250 ng/μL) was added to the reaction after 10 min. ([phis]) This lane was allowed to synthesize products for 60 min in the absence of heparin. (Right) (A) The RdRp products that were not able to resume RNA synthesis in the presence of heparin; (*) the RdRp products that were able. The vertical bar identifies the higher-molecular-weightproducts made by the HCV RdRp in the presence of heparin. Quantifications of the 13-nt product made at each time point were normalized to the amount made in the sample labeled [phis].
