Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p

TABLE 1.

S. cerevisiae strains used in this study

Effect on growth Strain Mutation Allele name Source
NA ANK800 None NA Kuhn and Brow 2000
NA PBY2 None NA This study, derivative of ANK800
Lethal PBY4 Domain (2178–2310) deletion None This study, derivative of ANK800
PBY5 F2206A, I2207A, Y2208A, G2209A prp8-604 This study, derivative of ANK800
PBY6 K2222A, T2223A, V2224A prp8-605 This study, derivative of ANK800
PBY8 L2256A, G2257A, W2258A prp8-606 This study, derivative of ANK800
Temperature sensitive PBY1 H2260A, Q2262A prp8-601 This study, derivative of ANK800
PBY7 P2228A, Q2229A prp8-608 This study, derivative of ANK800
PBY16 Q2202A, H2260A, Q2262A, E2273A prp8-602 This study, derivative of ANK800
PBY17 I2259A prp8-28 This study, derivative of ANK800
PBY18 D2198A, V2199A, K2200A prp8-607 This study, derivative of ANK800
PBY19 V2184A, L2185A prp8-603 This study, derivative of ANK800
No growth defects observed PBY10 E2273A, V2274A None This study, derivative of ANK800
PBY11 T2276A, H2277A None This study, derivative of ANK800
PBY12 M2269A, S2272A None This study, derivative of ANK800
PBY13 S2278A, K2279A, L2280A None This study, derivative of ANK800
PBY14 L2189A, L2190A, K2191A None This study, derivative of ANK800
PBY15 E2195A, I2196A, S2197A None This study, derivative of ANK800
PBY20 I2201A, Q2202A, V2203A None This study, derivative of ANK800
PBY21 D2214A, H2215A, P2216A None This study, derivative of ANK800
PBY22 P2186A, K2187A, N2188A None This study, derivative of ANK800
PBY23 V2225A, L2226A None This study, derivative of ANK800
PBY24 L2266A, K2267A, F2268A None This study, derivative of ANK800
PBY25 T2263A, E2264A, E2265A None This study, derivative of ANK800

This Article

  1. RNA 12: 292-302