The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells

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FIGURE 2.
FIGURE 2.

siRNA interactions with DNMT3A. (A) Western blot analysis following biotin/avidin pull-down shows that antisense and dsRNA EF52 associate with DNMT3A but not DNMT1. Flag-tagged DNMT1 and DNMT3A transfected 293T cell lysates were generated and incubated with a total of 500 nM biotin-labeled sense (S), anti-sense (AS), or double-stranded (S/AS) siRNA, and the control dsRNA without a biotin label (−). Immunostaining for the Western blot was performed with an anti-Flag antibody. (B) Western blot analysis showing comparable expression levels of both Flag-tagged DNMT1 and DNMT3A in the transfected 293T cell lysates.

This Article

  1. Published in Advance December 22, 2005, doi: 10.1261/rna.2235106 RNA 12: 256-262

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