Protein tagging at rare codons is caused by tmRNA action at the 3′ end of nonstop mRNA generated in response to ribosome stalling

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FIGURE 7.
FIGURE 7.

Northern blot analysis of crp mRNA derived from the crp genes encoding CRP variants. Total RNAs prepared from TA341 (Δcrp) and TA501 (Δcrp ΔssrA) cells harboring indicated plasmids were resolved by electrophoresis on a 2.0% agarose-formaldehyde gel. Northern blot analysis was performed using the DIG-labeled crp probe.

This Article

  1. Published in Advance December 22, 2005, doi: 10.1261/rna.2212606 RNA 12: 248-255

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