Protein tagging at rare codons is caused by tmRNA action at the 3′ end of nonstop mRNA generated in response to ribosome stalling

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FIGURE 5.
FIGURE 5.

Effect of overproduction of tRNAs corresponding to rare codons on SsrA tagging. Lysates equivalent to OD600 = 0.01 prepared from TA341 (Δcrp), TA501 (Δcrp ΔssrA), or TA481 (Δcrp ssrADD) cells harboring the indicated plasmid(s) were analyzed by Western blotting using anti-CRP antibodies. Plasmid pRARE contains the tRNA genes for AGA, AGG, AUA, GGA, CUA, and CCC rare codons (Novagen).

This Article

  1. Published in Advance December 22, 2005, doi: 10.1261/rna.2212606 RNA 12: 248-255

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