Protein tagging at rare codons is caused by tmRNA action at the 3′ end of nonstop mRNA generated in response to ribosome stalling

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FIGURE 2.
FIGURE 2.

SsrA tagging of CRP proteins expressed from crp variants. Lysates equivalent to OD600 = 0.01 prepared from TA341 (Δcrp ssrA+), TA501 (Δcrp ΔssrA), or TA481 (Δcrp ssrADD) cells harboring the indicated plasmids were analyzed by Western blotting using anti-CRP (lanes 1–9,11–13,15–20) and anti-DD-tag (lanes 10,14) antibodies.

This Article

  1. Published in Advance December 22, 2005, doi: 10.1261/rna.2212606 RNA 12: 248-255

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