The pseudouridine synthase RluD is required for normal ribosome assembly and function in Escherichia coli

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FIGURE 3.
FIGURE 3.

Ribosome profiles of wild-type and ΔrluD::kan mutant strains analyzed at various Mg++ concentrations. Ribosome profiles were performed as described in Materials and Methods. Wild-type and mutant cells were lysed, and gradients run at the Mg++ concentrations (mM) shown for lysis/sedimentation. Twelve A260 units were layered onto 14%–32% sucrose gradients and centrifuged at 22,000 rpm for 19 h at 4°C.

This Article

  1. Published in Advance May 31, 2005, doi: 10.1261/rna.2550105 RNA 11: 1141-1152

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