Impact of Paip2 inhibition of PABP on the translation of PLE-containing mRNAs. Capped control and +PLE mRNAs with 20-(A20) and 98- (A98) nt poly(A) were incubated on ice for 30 min with buffer (white bars) or 4 pmol or 8 pmol (light gray bars) of Gst-Paip2. Prior to the start of the reaction, 4 or 8 pmol of recombinant hPABP (dark gray and black bars, respectively) were added to mixtures containing 8 pmol of Gst-Paip2. Each bar represents the mean ± SD for triplicate determinations, and similar results were obtained for three independent repeats of this experiment. (B) The impact of Paip2 and added PABP on translation of each individual mRNA was determined as in Figure 7B by normalizing each data set to the values obtained for the buffer control, which for each mRNA was set to 1. The difference between control A20 versus +PLE A20 mRNA both with added Gst-Paip2 and Gst-Paip2+PABP was statistically significant, with p < 0.01 by Student’s T-test.
