Impact of PABP on poly(rA) inhibition of poly(A)-dependent translation. (A) Capped control and +PLE mRNAs with 20- (A20) and 98- (A98) nt poly(A) were translated as in Figure 6 in reactions containing 0, 50, or 100 ng of poly(rA). The reactions containing 100 ng of poly(rA) were also supplemented with 4 or 8 pmol of recombinant hPABP (dark gray and black bars, respectively). Each bar represents the mean ± SD for triplicate determinations, and similar results were obtained for three independent repeats of this experiment. (B) The fold effect of added PABP was determined as in Figure 6B by normalizing results for each mRNA to its corresponding buffer control. In each case the buffer control was arbitrarily set to 1. The difference between control A20 versus +PLE A20 mRNA was statistically significant, with p < 0.01 by Student’s T-test.
