mRNA with a <20-nt poly(A) tail imparted by the poly(A)-limiting element is translated as efficiently in vivo as long poly(A) mRNA

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FIGURE 2.
FIGURE 2.

Impact of a PLE on mRNA and protein expression in vivo. Cells were transfected with firefly luciferase (Fluc) constructs without (control) and with (+PLE) a PLE together with a plasmid expressing Renilla luciferase (Rluc). (A) RNA extracted 24 h after transfection was analyzed by Northern blot using [32P]-labeled probes to each mRNA. Radioactivity was visualized by PhosphorImager analysis. (B) Luciferase activity was measured in cytoplasmic extracts 24 h after transfection. The data are presented as the ratio of firefly (Fluc) to Renilla (Rluc) activity and show the mean ± standard deviation for triplicate determinations. Shown are typical results from three independent experiments.

This Article

  1. Published in Advance May 31, 2005, doi: 10.1261/rna.2470905 RNA 11: 1131-1140

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