TABLE 1.
Oligonucleotides
| Oligo | Sequence | Use | |
|---|---|---|---|
| Numbering of sequences (last column) are based on the following: oligonucleotides 4, 5, and 8–13, GenBank accession BC082438 (IMAGE: 50848776); oligonucleotides 14 and 15, GenBank accession BC076832 (IMAGE:6643643); oligonucleotides 16–23, indicated GenBank accession numbers; and oligonucleotides 24–33, sequences in the Unigene database. | |||
| 1 | DG1 | GCIAARGTICCIATHRTIAARTTY | degenerate PCR |
| 2 | DG2 | CCIYTIGTIYTNGTIRTIAARAARTGG | degenerate PCR |
| 3 | DG3 | RTCRAAIGGITCYTCIAYRCAIAY | degenerate PCR |
| 4 | xgld2-10 | TTGACTGGAGCAAAGACATC | Forward primer, XIGLD-2A (1448–1267) |
| 5 | xgld2-5 | TGGCCCACTTTTTAATGACC | Reverse primer, XIGLD-2A (1170–1189) |
| 6 | pVP16-1 | CTACGGCGCTCTGGATATGG | XIGLD-2 identification (pVP16 MCS) |
| 7 | pVP16-2 | CCTCTACAAATGTGGTATGG | XIGLD-2 identification (pVP16 MCS) |
| 8 | xgld2SmaN | CGCCCGGGGATGTACCCTAACTCCCCGAGCC | Forward primer, XIGLD-2A (178–199) |
| 9 | xgld2SmaC | CGCCCGGGTCATAAAGAGTTCATTTTTTTCACG | Reverse primer, XIGLD-2A (1683–1707) |
| 10 | xD242f | GAATCAGTGATGCAGcTTTGTGCCTGGTTTTAAAAGAG | Forward primer, XIGLD-2A active-site mutagenesis (887–924) |
| 11 | xD242r | CTCTTTTAAAACCAGGCACAAAgCTGCATCACTGATTC | Reverse primer, XIGLD-2A active-site mutagenesis (887–924) |
| 12 | Xlgld-2aNhel | CCGCTAGCGCTAGCATGTACCCTAACTCCCCCAGCC | Forward primer, XIGLD-2A (178–199) |
| 13 | Xlgld-2aXhol | CCCCTCGAGCTCGAGTCATAAAGAGTTCATTTTTTTCACG | Reverse primer, XIGLD-2A (1683–1707) |
| 14 | Xlgld-2bNhel | CCGCTAGCGCTAGCATGTACCCTAACTCCCCCAGCCTGGGCCGC | Forward primer, XIGLD-2B (139–168) |
| 15 | Xlgld-2bXhol | CCCCTCGAGCTCGAGTCATAACGAGTGCATTTTTTTCATGATTCC | Reverse primer, XIGLD-2B (1639–1568) |
| 16 | cycB1-74 | GCAGGTTTGCGCTTGAGAAAATGTCAC | Forward primer, cyclin B1 (accession no. J03166, 74–100) |
| 17 | cycB1-1010anti | GGAGGAAGCAGCTGCTATTTGGGAAGGCG | Reverse primer, cyclin B1 (accession no. J03166, 1010–1038) |
| 18 | cmos-361 | TACAGAGGGGAGACGGTGGCGCTG | Forward primer, cmos (accession no. X13311, 361–384) |
| 19 | cmos-3′anti | CCACTTAAACAGCAATGCAACAC | Reverse primer, cmos (accession no. X13311, 3073–3095) |
| 20 | Actin-1801 | CAACTGGAATAAGGGCAGACTTCC | Forward primer, actin (accession no. M24769, 1801–1824) |
| 21 | Actin-2227anti | GGGCCTCAGTATCAATTTCCAACC | Reverse primer, actin (accession no. M24769, 2227–2251) |
| 22 | L1-1033 | GCTGAACCCATATGCAAAGACCGC | Forward primer, L1 (accession no. BC054956, 1033–1056) |
| 23 | L1-3′anti | ACAGAATTTATTGAGTAAATCC | Reverse primer, L1 (accession no. BC054956, 1279–1300) |
| 24 | GLD-2(L) F | TTATGGCATGACTTTTCAGCA | Forward primer, mGLD-2(L) (1907–1928) |
| 25 | GLD-2(L) R | GGCCAGTGAATTGTAATAGCACTCACTATAGGGAGGCGTCCGTTTTCTGCTGTTTCCT | Reverse primer, mGLD-2(L) probe (2484–2504) |
| 26 | CPEB-1 F | CATCTTGGGACCTTCTTGGA | Forward primer, CPEB1 (808–828) |
| 27 | CPEB-1 R | AAGACCCAAGGGATTACC | Reverse primer, CPEB1 (1246–1266) |
| 28 | CPEB-2 F | GGTCGCTCTTCCCTATTTCC | Forward primer, CPEB2 (773–793) |
| 29 | CPEB-2 R | TCCAAAGGCTGAGAACCATC | Reverse primer, CPEB2 (1198–1218) |
| 30 | CPEB-3 F | CCCTTCTCCAGCAATGTGAT | Forward primer, CPEB3 (958–978) |
| 31 | CPEB-3 R | CTCGTTCCCCATTTTGACAT | Reverse primer, CPEB3 (1412–1432) |
| 32 | CPEB-4 F | CCTCACTGCTTCACTCACCA | Forward primer, CPEB4 (97–117) |
| 33 | CPEB-4 R | AACAAAGCGGGCACTGATAG | Reverse primer, CPEB4 (643–663) |
