mRNAs encoding CPEB isoforms are differentially expressed in mouse cerebellum. Darkfield microscopy of 35S-labeled RNA probes on emulsion-coated sections. (A–E) Bar, 100 μm. (A) mGLD2 mRNA is found in the major neuronal cell populations in the cerebellum, including the granule cell layer (GCL) and the Purkinje cell layer (PCL), with less prominent expression in the cell-sparse molecular cell layer (MCL). (B) mCPEB1 expression was confined to Purkinje neurons (arrows). (C) mCPEB2 was expressed in a similar pattern to mGLD-2. (D) mCPEB3 was expressed in a similar pattern to mGLD-2 except that more prominent labeling was evident in cells of the MCL. (E) mCPEB4 was expressed in a similar pattern to mGLD-2 except that more prominent labeling was present in cells within the PCL.
