The distribution of mGLD2 in hippocampus is nearly identical to those of mCPEB1, mPumilio1, and ARC. Darkfield microscopy of 35S-labeled RNA probes on radiographic emulsion-coated sections. (A–G) Bar, 200 μm. (A–E) mGLD2 mRNA was detected in the pyramidal cell layers (CA1 through CA3) of the hippocampus and granule cell layers (GCL) of the dentate gyrus (A). Nearly identical distributions were detected in adjacent sections hybridized with probes complementary to mCPEB1 (B), Pumilio1 (C), or ARC (D). The mRNA for the RNA binding protein Quaking, which is expressed primarily in glial cells, was more abundant and distributed differently than mGLD2 (E). (F, G) Three hours of seizure activity induced by pentylenetetrazole (PTZ; 50 mg/kg intraperitoneal), increased ARC expression in dendritic and cellular layers of the dentate gyrus (F). (The dark area over the granule cell layer is overexposed silver grains). In contrast, PTZ treatment did not change the abundance or distribution of mGLD2 mRNA (G).
