FIGURE 5.
XlGLD-2 interacts with target, but not nontarget, mRNAs. Oocyte extracts were incubated with α-XlGLD-2 or preimmune guinea pig serum and bound to Protein A–Sepharose. Bound material was eluted and the RNA recovered. mRNAs were detected by semiquantitative PCR, using oligo(dT)-primed reverse transcription reactions, followed by PCR using gene-specific primers. Cyclin B1 mRNA receives poly(A) during oocyte maturation, and is immuno-precipitated by α-XlGLD-2. Cytoskeletal actin and ribosomal protein L1 mRNAs do not receive poly(A) during maturation and are not immunoprecipitated.
