A summary of the RNA residues that are known to cross-link to Prp8 in the context of the spliceosome. Cross-linked residues are circled, the exons are boxed, and the intron is represented by a thin blue line. (Red circles) Short-range cross-links using uridine and 4-thiouridine; (green circles) long-range cross-links using the reagent benzophenone. Where both cross-linking agents were used at the same site the circles colored red and green with a dotted outline. Extensive Prp8 cross-links with U5 snRNA are also represented as is the U6 residue 54. Both the U5 and U6 cross-links were only demonstrated in snRNPs. All numbering relates to S. cerevisiae.
