FIGURE 2.
Maps showing regions of Prp8p against which antibodies have been generated. The regions recognized are represented as arrows below the antibody names. S. cerevisiae antibodies 8.1–8.4 were raised against lacZ–protein fusions (Lossky et al. 1987; Jackson et al. 1988) whereas all the others are raised against peptides. α-8.1 only precipitates yPrp8 in U5 snRNPs, whereas α-8.4 and α-8.6 are the only ones that precipitate yeast spliceosomes and co-complexes. The 70R antibody against hPrp8 (Luo et al. 1999) is noted as being superior to KO5 for immunoprecipitation. One other antibody to tbPrp8 is known (Lucke et al. 1997).
