FIGURE 3.
Northern analysis of GlnAGGU and GlnQSerC44 mutant tRNAs. Low molecular weight RNAs were prepared from XAC/A16 cells expressing the plasmids pGFIB, pGFIB tRNAGln, pGFIB GlnAGGU, or pGFIB GlnQSerC44. The high background on the films on the right reflects prolonged exposure. Identification of tRNA and precursor tRNA bands were based on gel mobility (Comer et al. 1974; Seidman et al. 1974; McClain and Seidman 1987). The probes for GlnQSerC44 showed precursor tRNAs, while the probes for GlnAGGU crosshybridized with wild-type tRNAGln transcribed from the XAC/A16 chromosome, and showed no signal of overexpressed GlnAGGU tRNA.
